Tomato Genome-Wide Transcriptional Responses to Fusarium Wilt and Tomato Mosaic Virus

摘要

Since gene expression approaches constitute a starting point for investigating plant–pathogen systems, we performed a\udtranscriptional analysis to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol)\udand Tomato Mosaic Virus (ToMV). Differentially expressed tomato genes upon inoculation with Fol and ToMV were identified\udat two days post-inoculation. A large overlap was found in differentially expressed genes throughout the two incompatible\udinteractions. However, Gene Ontology enrichment analysis evidenced specific categories in both interactions. Response to\udToMV seems more multifaceted, since more than 70 specific categories were enriched versus the 30 detected in Fol\udinteraction. In particular, the virus stimulated the production of an invertase enzyme that is able to redirect the flux of\udcarbohydrates, whereas Fol induced a homeostatic response to prevent the fungus from killing cells. Genomic mapping of\udtranscripts suggested that specific genomic regions are involved in resistance response to pathogen. Coordinated\udmachinery could play an important role in prompting the response, since 60% of pathogen receptor genes (NB-ARC-LRR,\udRLP, RLK) were differentially regulated during both interactions. Assessment of genomic gene expression patterns could\udhelp in building up models of mediated resistance responses.